BIO lab 1408 microscopy and cytology report
MICROSCOPY AND CYTOLOGY REPORT SHEET
Bio Lab 1408
- Microscopy
Experiment (dry lab). See the powerpoint slides to complete worksheet
- Locate the parts below and match them to their description.
ocular lens head
objective lens stage
condenser lens with filter light
revolving nosepiece base
coarse adjustment knob rheostat
fine adjustment knob arm
iris diaphragm
|
A |
Eyepiece |
ocular lens |
|
B |
Supports the ocular lenses |
head |
|
C |
Holds objectives that can be rotated |
revolving nosepiece |
|
D |
Connects the base and barrel |
arm |
|
E |
Lens that magnifies the specimen |
objective lens |
|
F |
Supports the slide |
stage |
|
G |
Focuses the light onto the specimen |
condenser lens with filter |
|
H |
Raises and lowers the stage for focusing |
coarse adjustment knob |
|
I |
Slightly moves the stage to sharpen the image |
fine adjustment knob |
|
J |
Illuminator |
light |
|
K |
Light control |
rheostat |
|
L |
Supports the microscope |
base |
|
M |
Varies the intensity of the light |
Iris diaphragm |
- Principles of microscopy
Experiment (dry lab)
Field of view
|
Lens |
Lens Power |
Ocular lens |
Total Magnification |
Diameter in mm (3 significant figures) |
Diameter in mm (3 sig figs) |
Area in mm2 A = pr2 (p = 3.14) (3 sig figs) |
|
Scan Power |
4X |
10X |
40X |
4.200 |
4200 |
13.85 |
|
Low Power |
10X |
10x |
100x |
1.680 |
1680 |
22.16 |
|
High Power |
40X |
10x |
400x |
0.420 |
420 |
13.85 |
|
Oil Immersion |
100X |
10x |
1000x0 |
0.168 |
168 |
26.73 |
Note:
- power of ocular lens does not change
- to determine total magnification use: lens power X ocular lens power
- to determine diameter in mm: 40x X 4.200/total mag = diameter in mm
- to convert mm to mm: mm X 1000 = mm
- to determine area: radius is ½ of diameter. Take radius and square it then multiply by p (pi)
What happens to the field of view as magnification increases?
|
Decreases |
(It increase, decrease or stay the same.)
-Depth of focus
What happens to the depth of focus as magnification increases?
|
Decreases |
(It increase, decrease or stay the same.).
-Image orientation
Select the letter ‘e’ as you would see through the ocular if it were mounted right side up on the slide. (A, B, C or D.)
|
A |
- Cytology
Experiment (dry lab)
- Wet Mounts
- Eukaryotes
- Elodea leaf cells:
- Label the cell - plasma membrane, cell wall, chloroplast, and cytoplasm
|
A |
Cell wall |
|
B |
Plasma membrane |
|
C |
Chloroplast |
|
D |
Cytoplasm |
- Onion cells.
- Label the cell - plasma membrane, cell wall, cytoplasm, nucleolus, and nucleus
|
A |
Cytoplasm |
|
B |
Cell membrane |
|
C |
Cell wall |
|
D |
Nucleolus |
|
E |
Nucleus |
- Human cheek cells.
- Label the cell - plasma membrane, cytoplasm and nucleus
|
A |
Plasma membrane |
|
B |
Nucleus |
|
C |
Cytoplasm |
- Prokaryotes
Identify these bacterial cells as coccus, bacillus or spirillum
|
A |
Coccus |
|
B |
Bacillus |
|
C |
Spirillum |
- Cell Models: Label the animal and plant cells.
Animal cell model:
Label - centrioles (pair), cytoplasm, Golgi apparatus, mitochondria, nucleus and plasma membrane.
|
Animal cell model #1 | ||
|
A |
Boundary between cytosol and extracellular fluid |
Plasma membrane |
|
B |
Flattened stacked discs |
Centrioles (pair) |
|
C |
Synthesize ATP |
Golgi apparatus |
|
D |
Cytosol + organelles |
Mitochondria |
|
E |
Found in pairs, made of microtubules |
Nucleus |
|
F |
Holds the DNA |
Cytoplasm |
Nucleus:
Label - DNA (chromatin), nuclear membrane, nuclear pore and nucleolus.
|
Animal cell/Plant cell Model #2 | ||
|
A |
Double membrane |
Nuclear pore |
|
B |
Assemble ribosomal subunits |
Nucleolus |
|
C |
Genetic material |
DNA (Chromatin) |
|
D |
Perforation on the nuclear membrane |
Nuclear membrane |
Plant cell:
Label – cell wall, central vacuole, chloroplasts, cytoplasm, Golgi apparatus, plasma membrane, mitochondria and nucleus.
|
Plant cell Model #3 | ||
|
A |
Boundary between cytosol and cell wall |
Plasma membrane |
|
B |
Synthesis of ATP |
Golgi apparatus |
|
C |
Flattened stacked discs |
Central vacuole |
|
D |
Supports the cell |
Cell wall |
|
E |
Holds the DNA |
Nucleus |
|
F |
Cytosol + organelles |
Mitochondria |
|
G |
Membranous sac stores water + other substances |
Cytoplasm |
|
H |
Site for photosynthesis |
Chloroplasts |
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