Be6056 - Database - Assessment Answer

BE6056 - Database - Steroids - Fatty Acids - Xenobiotics - Bioinformatics Assessment Answer

Assessment Task:

Question 

Hexokinase is an important enzyme which is part of the glycolytic pathway.  There are several forms of the enzyme, one of which is highly expressed in cancer cells.  

1. Use the NCBI or EBI portals to retrieve one file for each of the several different forms of hexokinase found in humans.  Each file should contain the complete mRNA sequence (it is not necessary to print the sequence out).

2. Compile a table, similar to the one from tutorial 1 (page 6), comparing the sequence elements of the mRNA of each different hexokinase forms that you find.  Include extra columns indicating the length of the protein and the receptor sub-type.  Comment on your findings.

3. Retrieve files for two genes of the hexokinase types you retrieved in part (a) and compare the structure of the genes (exon / intron profile).   Comment on your findings.

For all parts describe how you obtained your data by stating the bioinformatics portal used and the search strategy.  Accession numbers of all sequence files must be given.  Any references used should be cited in your answer.  Expect to retrieve about a dozen files in total.

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